FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787939

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787939
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106361005
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGACAT406900.030.7326227
TGCGACA406150.030.6306656
TGGCGCG705000.026.5564924
CGACATC527300.023.7257128
AGGGCGA536600.022.5395574
GGGCGAT261350.022.4698051
GCTGCGA582200.021.5825564
CTGCGAC586950.021.4699615
GGCGATC281650.021.1192672
GTGGCGC949050.020.7961143
CAATCGC198550.019.9494881
CGGTGGC1402250.018.1241471
GGCGCGT971950.018.0720835
TCAATCG158550.017.6087933
CTCGCGT172900.017.5953738
CGATCTG333600.017.567144
TCGCGTC175550.017.4769959
CGTGCCT1123150.016.2408719
GCGAGGC777800.015.5222787
GGGCGAG816900.015.1738575
Produced by FastQC (version 0.11.5)