FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787940

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787940
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114215779
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2020770.17692564177144035No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1684130.1474516056139669No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1591240.13931875384748724No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1574390.13784347607522776No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1386420.12138603020866319No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1219340.10675757856539243No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT390800.054.368596
CCGCGTT422950.050.2658547
AGACCGC427800.049.279544
TCGACCG613900.047.459095145
CAGACCG468300.045.7090263
CTCGCTA649250.039.9440771
TCGCTAT726700.035.5898132
GCGTTCT597350.035.4633679
GTGCTCT1025950.033.54504145
CGCTATG786850.033.0611953
ACCGCTA139150.029.7913282
CGCGTTC759550.027.7728798
CGATCCT1367650.026.65942145
CCGGTAA157100.025.069911
CTTCGAC853800.023.823702145
GACCGCG978150.021.8646095
CCGCTAA219550.021.6275183
GTCTCGC645600.021.4157314
CGGTAAT166450.021.396312
TCGCGTC305000.020.1478259
Produced by FastQC (version 0.11.5)