FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787941

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787941
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114215779
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1606600.14066357679003355No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCGTA199150.027.815449
AGGGCGA672750.026.3206234
CAATCGC307350.023.85751
TCAATCG227300.023.7264583
TGCGACA350750.023.6716656
CTACCGT240850.023.0261028
GCGACAT374000.022.4043947
GGGCGAT238250.022.324111
CGGTGGC1697250.022.019761
GGCGATC261800.020.5999832
ACTACCG271350.020.5083397
CGACCGT82750.020.3588071
CGGAGGG977650.018.5789221
CGACATC487700.017.3247558
GGGCGAG1043750.017.29445
TGGCGCG1027650.017.1502233
GTGGCGC1391150.016.7332823
GGGGACC4410600.016.599499145
GGCGAGG1087800.016.5179776
GAGGGCG1106400.016.3956723
Produced by FastQC (version 0.11.5)