FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787942

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787942
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113532512
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2244850.19772750205685574No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1931750.17014949867400098No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1435780.12646421493783208No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1389300.12237023347109593No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1216770.1071737054492373No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1180650.10399223792388211No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT488350.060.1545226
CCGCGTT512750.057.243057
AGACCGC541950.054.146464
CAGACCG574700.051.3992423
TCGACCG607350.050.039898145
GCGTTCT637350.045.8849079
CGCGTTC679450.043.0417868
GTGCTCT1171700.042.246464145
CTCGCTA553800.042.211021
GACCGCG706950.041.5975575
TCGCTAT594250.038.7206762
CGCTATG640250.035.832013
CTTCGAC810800.027.588734145
CGATCCT1225650.027.472755145
TCAGACC1172850.026.5114352
CTCAGAC1433850.024.8719861
ACCGCTA178800.023.3439222
CCGCTAA205500.021.9734233
GCTATGT1140650.020.5029474
CGGTGCT767300.019.958855145
Produced by FastQC (version 0.11.5)