FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787943

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787943
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113532512
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1294500.026.375541
GCGACAT335000.026.2387377
TGGCGCG844550.024.935564
CAATCGC196550.024.7601811
TGCGACA358350.024.1589766
TCAATCG136650.023.2897383
GGCGCGT930250.020.8666575
GTGGCGC1109500.020.1132833
CGACATC447500.019.8126458
GGCGATC253100.018.2626022
GGGCGAT257350.017.9649621
GGGGACC3426850.017.094408145
CTGCGAC523700.017.0617085
CGTGCCT1281050.017.0606549
GGTGGCG1541150.016.8112622
GCTGCGA537850.016.4015124
AGGGCGA614750.016.3322964
AATCGCC305000.016.252252
CGGAGGG646350.016.0991
GCGTGCC1192250.015.8793498
Produced by FastQC (version 0.11.5)