FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787944

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787944
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112353801
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1570990.1398252650126185No Hit
CTGGAGTCTTGGAAGCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGC1263900.11249285638320328No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1205500.1072949904026834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT303650.053.9048966
CCGCGTT343900.047.7852367
AGACCGC343700.047.270124
CAGACCG391450.042.217863
CTCGCTA351550.041.821021
TCGCTAT392950.036.774962
CGCTATG418550.034.360563
GCGTTCT516500.031.7539989
ACCGCTA193100.026.6306252
CGATCCT1053200.025.225542145
CCGCTAA223850.025.0251313
CGCGTTC686800.023.672168
GAGTCTT1253750.021.8687924
GACCGCG767700.021.4411325
CGCTAAG261100.020.6335454
CGTATCG114750.020.6145973
AGTCTTG1392300.020.2810065
TCGACCG656250.020.254723145
GGAGTCT1388850.019.6947173
CGCTAGA328950.019.5537411
Produced by FastQC (version 0.11.5)