FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787945

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787945
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences112353801
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA708600.026.6981334
TGCGACA346350.026.5485536
GCGACAT356550.026.173197
TCAATCG210900.024.4417463
CAATCGC271350.023.9370651
TGGCGCG834050.023.565374
CGACATC488150.019.4769348
TACCGTA198400.019.2807259
CTACCGT214400.017.9327058
GTGGCGC1170150.017.877053
GCTGCGA534100.017.6311384
CTGCGAC558300.016.9719525
GGGCGAG1182550.016.8300175
GGTCACG610700.016.568197145
GGCGAGG1195900.016.5656226
GCGAGGC1158400.016.4157527
CGGAGGG1258650.016.2354281
CGACCGT105250.016.0114121
AATCGCC412800.015.7286412
CGGTGGC1649600.015.5288461
Produced by FastQC (version 0.11.5)