FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787946

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787946
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149949229
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2297720.15323319868486954No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA2159570.144020080289976No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2149210.143329179771908No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2029460.13534314337821637No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1919970.12804133857867317No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT476250.059.656246
CCGCGTT515950.055.017257
AGACCGC561950.050.3114364
TCGACCG816050.050.22795145
CAGACCG589700.048.6683773
GCGTTCT668800.042.509769
CTCGCTA914600.039.4457131
TCGCTAT1013850.035.640842
CGCTATG1108900.032.5838853
CGCGTTC883050.032.0175068
ACCGCTA217650.030.9467032
CTTCGAC1037850.026.768988145
CGATCCT1794250.025.592241145
GTGCTCT1317650.025.524311145
GACCGCG1118950.025.5201325
CCGCTAA312650.023.5114423
GTCGCGT81750.022.131481
GCTATGT1688400.021.7128774
TCAGACC1420700.021.4229852
CTCAGAC1749850.020.417691
Produced by FastQC (version 0.11.5)