FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787947

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787947
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences149949229
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1891830.12616470338770464No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG211050.032.387863
TGGCGCG1061450.027.4936354
CAATCGC309850.026.755961
GCGACAT430350.025.59827
TGCGACA430350.025.158626
CGGTGGC2161650.021.6036111
GTGGCGC1531400.020.4482633
AGGGCGA777600.019.940714
GGGCGAT317400.019.6349471
CGACATC599500.018.6491018
GGCGATC333800.018.6077522
AATCGCC446500.018.4162042
CTGCGAC635450.017.316195
GCTGCGA676200.016.393894
GGGGACC5587150.016.354893145
GGTCACG693050.016.235825145
ATATCGG180250.015.606433145
ACGCCCG1037000.015.3600626
CGGCCGC3525750.015.095107145
GGCGCGT1666400.014.9811325
Produced by FastQC (version 0.11.5)