FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787948

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787948
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136220833
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA3108400.22818829774737906No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1954320.1434670422254722No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1591670.11684482945424361No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1537010.11283222735835127No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT613550.065.89476
AGACCGC646950.062.2569164
CCGCGTT668200.060.4675067
CAGACCG709050.057.3842433
TCGACCG589150.052.653305145
GTGCTCT1403300.052.46201145
GCGTTCT882050.045.879669
CTCGCTA574400.039.528631
TCGCTAT635350.035.427592
ACCGCTA208050.033.4786762
CGCGTTC1214200.033.203618
CGCTATG689550.032.769453
GACCGCG1294450.031.3308125
TCAGACC1481250.028.9781192
CTCAGAC1804900.026.639951
CCGCTAA306200.024.5847193
CGATCCT1125700.023.553112145
GTCGCGT71850.022.9533541
CGCTAAG353350.020.928254
CTTCGAC851300.020.64519145
Produced by FastQC (version 0.11.5)