FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787949

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787949
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136220833
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1845690.13549249107880584No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC348050.031.7857361
TCAATCG234150.031.0274643
AGGGCGA870350.029.4598054
GCGACAT364500.024.3009267
CGGAGGG1122450.023.7177721
TGCGACA382300.022.9537056
TACCGTA189600.021.8863059
AATCGCC526950.020.9878162
GGTCACG805550.018.533142145
GGGCGAG1445450.018.3005335
GAGGGCG1456700.018.1112863
GCGAGGC1442650.017.6499357
CTACCGT238350.017.5164328
GGCGAGG1546850.017.3387996
CGACATC543700.016.548248
GCTGCGA567550.015.7745814
GGGCGAT259800.015.6467241
GGCGATC281200.015.3212342
CGGTGGC1951900.014.9538291
CGTTCTT710650.014.874313145
Produced by FastQC (version 0.11.5)