FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787950

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787950
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105742363
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2244920.2123009110360055No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1538190.14546582432624472No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1411210.13345739209554072No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1308660.12375929219588179No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1245290.11776642441780878No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1227650.1160982188377992No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG558050.048.288788145
ACCGCGT224350.040.1266946
CTCGCTA485300.039.6764641
TCGCTAT497400.038.367572
CGCTATG535450.035.8489463
CCGCGTT266600.033.6971327
AGACCGC270550.032.6501964
ACCGCTA173550.032.4480172
CAGACCG303500.029.8297653
CCGCTAA230100.026.6198793
CTTCGAC773250.026.188553145
CGATCCT1238400.024.191977145
CGCTAAG267650.022.1818394
GCGTTCT407700.021.8815299
GCTATGT1008550.019.3843484
GTGCTCT926000.019.215242145
CGCGTTC475500.018.6167878
TCGTACG183150.018.40927310-14
GTCGCGT57300.018.2569311
CGTATCG78900.017.1283973
Produced by FastQC (version 0.11.5)