FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787951

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787951
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105742363
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1955030.18488616525431723No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG151500.026.4822163
CAATCGC216200.025.5989381
AGGGCGA654200.024.2813954
GCGACAT341750.023.2388467
TGCGACA343750.022.8477636
CGGTGGC1489700.022.5090641
GGGGACC3391450.021.653908145
GGCGATC240300.021.1408982
GGGCGAT241650.020.8999021
CGGAGGG787900.020.7739891
TACCGTA141100.017.6164429
GGGCGAG967100.017.11895
GCGAGGC939900.017.0463587
CGGCCGC2508250.016.92097145
CGACATC482100.016.887038
TGGCGCG838550.016.4874863
ACGCCCG637450.016.3391466
GGCGAGG1021950.016.2120846
AATCGCC351850.015.86622
GAGGGCG1036300.015.7102513
Produced by FastQC (version 0.11.5)