FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787952

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787952
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104017606
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2143780.20609780232780975No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1462480.14059927508810383No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1307420.1256921833021229No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT459700.059.189096
AGACCGC495800.054.6687934
CCGCGTT530800.051.307087
CAGACCG545750.050.447083
GCGTTCT623050.043.6316159
TCGACCG353200.037.410557145
GACCGCG743300.036.6638375
CGCGTTC750550.036.26068
GAACGTA455700.036.022015
AACGTAT478200.034.314276
CGCTATG579550.032.832593
CGTATTC515000.031.8146178
TCGCTAT646200.029.8283162
CGGCGTG358250.029.8144145
CGACCTA80050.028.702574
GTGCTCT1118500.028.319555145
GGTGCTC992750.028.142118145
AGAACGT384400.027.0412944
CTCAGAC1235250.025.9185181
TCAGACC1163250.024.5988242
Produced by FastQC (version 0.11.5)