FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787953

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787953
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences104017606
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1302050.1251759245449275No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG151900.027.399813
CAATCGC205500.025.1105671
TTGATCC840350.021.8800853
GTTTGAT895500.021.5827751
TGGCGCG734100.021.334344
TACGCTG296800.020.923042145
TTTGATC952500.019.9412672
CGCACAA257150.019.3372631
CGGTGGC1392550.018.4605831
CGGCCGC2311600.017.891994145
GCGACAT233350.017.7818957
GGGGACC3023400.017.572813145
ACGCCCG677300.016.9861896
CTAACGC235500.016.976282145
GTGGCGC993200.016.9545613
GATCCTG1194750.016.8337255
AATCGCC314700.016.2843822
TGATCCT1199800.016.270574
TCGCGTC124800.016.2506459
CTCGCGT127000.015.9206078
Produced by FastQC (version 0.11.5)