FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787954

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787954
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117967577
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1831720.15527317306856273No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG1808760.15332687557022553No Hit
GGCCCGGCTCGCGTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCC1645420.13948069815827446No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1415610.11999992167339335No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1307830.1108635129464429No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG699700.054.111774145
ACCGCGT272050.049.6785966
CCGCGTT302700.044.518917
AGACCGC317000.042.3505974
CTCGCTA405900.039.215321
CAGACCG374300.036.784533
TCGCTAT443200.035.449062
CGCTATG454200.034.500753
CGGCGCT1187850.031.041084145
ACCGCTA211750.029.0868432
CTTCGAC992150.028.467373145
GCGTTCT492800.027.2796579
CGCGTTC511650.026.2108978
CCGCTAA257000.024.962223
CGATCCT1020750.024.857527145
GTCGGCT303100.024.5015661
TTCGGCG350750.023.843167
GGTGCTC972500.023.563164145
GACCGCG599050.022.6064435
CGCTAAG283900.022.1642254
Produced by FastQC (version 0.11.5)