FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787955

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787955
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117967577
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA403600.029.6606436
GCGACAT427850.028.362017
AGGGCGA654100.026.1263894
CAATCGC293150.022.3201031
CGACATC551250.022.1314338
CTCGCGT268950.021.8784988
TCGCGTC274650.021.6394889
TACCGTA181800.021.6144059
TGGCGCG964600.021.5675354
TCAATCG214450.020.4946963
GCTGCGA602700.020.2160244
CTGCGAC622450.019.5369855
GGGCGAT332050.019.2446881
CTACCGT221750.018.1619978
GGCGATC362600.017.8399522
GTGGCGC1239950.017.4907573
GCGAGGC992650.017.2829657
GGGCGAG1040250.017.1142945
CGGTGGC1743300.016.4931851
GGCGAGG1112000.016.150926
Produced by FastQC (version 0.11.5)