FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787956

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787956
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130659866
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1470570.11254948019003784No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT449100.046.13676
CCGCGTT489900.042.294737
AGACCGC482400.042.1416444
TCGACCG654400.041.27005145
CAGACCG530300.038.88493
CTCGCTA494450.037.431151
TCGCTAT521000.035.3691442
CGCTATG548700.033.591833
GCGTTCT651900.031.453959
ACCGCTA313150.029.1721132
CTTCGAC967800.027.514324145
GGTGCTC1147800.026.212397145
CGCTAAG372050.025.3984324
CCGCTAA378400.025.2787463
TCGCGTC512650.024.9635669
CTCGCGT527700.024.3381778
TCGGTGC626400.023.686857145
TCGTACG268950.020.44083810-14
CGTATCG118450.019.9682063
CGATCCT888650.019.643078145
Produced by FastQC (version 0.11.5)