FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787957

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787957
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences130659866
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGAGGGCGAGGCCCGCGGCGGCGGCGGCGCCGCCGCGCGCTTCCCTCCG1374360.1051860867513824No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA984350.039.633444
CAATCGC319100.032.108321
CGGAGGG1463000.027.6795371
TCAATCG208900.026.1287563
GAGGGCG1563750.025.4536083
GGGCGAG1604150.025.1845345
GCGAGGC1589550.024.4787867
GGGCGAT305350.023.6865631
GGCGAGG1722350.023.490336
GGCGATC319750.022.8820552
GCGACAT397500.022.8776557
TGCGACA412650.021.689436
AATCGCC483450.021.1253992
TCGCATA108700.020.1337497
CGGCCGC4577650.019.889761145
CGAGGCC2047300.019.3270348
CTCGCGT171350.017.9566768
TCGCGTC171050.017.8736089
GGAGGGC2327650.017.6211032
TACCGTA206200.016.8226859
Produced by FastQC (version 0.11.5)