FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787959

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787959
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99567909
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA661200.033.3306544
TGCGACA312150.029.4078836
TACCGTA157350.028.513589
GCGACAT329050.027.9764867
CTACCGT191100.023.578278
TCAATCG243350.022.8803163
CAATCGC315850.022.4607751
CGACATC426850.021.8994338
CTCGCGT283950.021.559968
ACTACCG215700.021.4289557
GTCGGTC195100.021.2645531
TCGCGTC288950.021.2101969
GGGCGAG1128650.020.4767075
TGGCGCG947500.020.4747754
GCTGCGA473000.019.6185934
GCGAGGC1148900.019.2099557
GGCGAGG1227800.018.7070946
ACGATAC85150.018.5245865
CGACCGT81600.018.4514081
CTGCGAC530100.017.8048535
Produced by FastQC (version 0.11.5)