FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787961

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787961
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences105011818
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA718100.034.2114834
TGGCGCG1015250.027.5651764
TGCGACA310850.026.8742356
GCGACAT315900.026.6721237
TCAATCG201550.024.9228133
CAATCGC278900.024.5922931
TCGCGTC243900.022.3003229
CTCGCGT244700.022.1464488
GGGCGAG1169600.021.9797485
GTGGCGC1342550.021.8515153
GGCGAGG1242400.020.7125456
GCGAGGC1194350.020.5960887
TACCGTA157300.020.5641069
CGGTGGC1826850.020.4387971
CGACATC434100.019.664688
CTACCGT170750.018.5614788
GCTGCGA483700.017.4650634
AATCGCC399000.017.2826962
CTGCGAC500950.017.105845
CGTGCCT1631000.016.8938889
Produced by FastQC (version 0.11.5)