FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787962

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787962
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143502159
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT2507100.17470817285752474No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC2477690.1726587263401382No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1677330.11688534943923734No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1585870.11051192616551503No Hit
GTCCAGAGTCGCCGCCGCCGCCGGCCCCCCGGGTGCCCGGGCCCCCCTCG1548950.10793914257415459No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1451600.10115527251405325No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG1231550.043.75461145
CTCGCTA533650.040.7063981
TCGCTAT558600.038.4387932
CGCTATG592200.036.1732673
ACCGCGT296650.035.3323636
ACCGCTA236950.034.775872
CTTCGAC1646750.031.820137145
CCGCGTT336300.031.245117
AGACCGC351500.029.3364454
CCGCTAA323200.027.8226413
CAGACCG406500.026.1786753
TCGCGTC993500.024.8776729
CGCTAAG352300.024.7941474
CTCGCGT999750.024.656178
CGTATCG201550.023.7971783
GTCTGGA1767750.022.2285861
CGATCCT1349350.022.158352145
CCGGTAA178550.021.9814831
CGGCGCT1169600.020.97294145
CCCGTAT332950.020.9140831
Produced by FastQC (version 0.11.5)