FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787963

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787963
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences143502159
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGCGAT445400.034.503771
GGCGATC489200.031.4071852
AGGGCGA832700.030.2936154
TGCGACA711000.028.9027186
GCGACAT759250.027.27497
TACCGTA224350.025.6312089
CGACATC863550.024.0563268
CAATCGC403050.023.6732651
CGATCTG661800.023.4732064
GCTGCGA904950.022.9808144
CTCGCGT257150.022.2589058
TCGCGTC260750.022.078519
CTACCGT278650.020.8498158
CTGCGAC1006500.020.8265535
CGACCGT122900.020.6595361
TGGCGCG1191400.020.0466444
GCGATCT789500.019.902533
GCGAGGC1288600.019.732937
CGGTGGC2153450.019.5383451
GGGCGAG1350800.019.4282825
Produced by FastQC (version 0.11.5)