FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787965

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787965
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences147562295
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGGCGCG1266350.029.5291944
CGGTGGC2219350.027.2063921
CAATCGC299450.023.5115471
GTGGCGC1691150.023.5038013
CTCGCGT291250.023.2076288
TCGCGTC294700.022.8024759
AGGGCGA886300.021.7545094
TCAATCG210900.020.7787363
GGCGCGT1667050.020.706625
GGTGGCG2526150.019.2868442
GGGGACC6315450.018.99898145
CGTGCCT2022500.017.8089549
GCGTGCC1999800.016.7620548
GCGAGGC1187800.016.1892687
ACGCCCG1499600.015.7830626
GGGCGAG1332150.015.1732955
GGCGAGG1338700.014.9227436
GCGACAT304800.014.8850257
AATCGCC471600.014.8461722
CGCGTGC2291650.014.616597
Produced by FastQC (version 0.11.5)