FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787966

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787966
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165537406
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2354260.142219215395945No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT2116390.127849653509733No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1831910.11066441381834871No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1793550.1083471127969711No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG731200.044.699165145
ACCGCGT340700.035.982046
CGCTATG792600.033.258553
TCGCTAT802000.033.229052
CTCGCTA858100.032.2216761
CCGCGTT397350.030.8679527
AGACCGC412500.029.5363274
CTTCGAC1050400.027.303314145
CGGTTCG385850.025.652174145
CAGACCG495150.025.3155423
TCGCGTC695850.025.0108099
GAACGTA395200.024.0725145
CGGAGTA547250.023.9644174
CTCGCAT391550.023.753771
AACGTAT402500.023.5243556
CGATCCT1962100.021.666565145
CGTATCG165850.021.4505523
GAGTACC595450.021.0296366
CGTATTC455700.020.749718
GCGTTCT617750.020.0522489
Produced by FastQC (version 0.11.5)