FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787967

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787967
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences165537406
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC2163100.023.6250231
TCAATCG272450.023.519383
TGGCGCG1442650.023.2298684
GCGACAT499600.022.0910037
TGCGACA496050.021.9770666
AGGGCGA998550.021.951074
CAATCGC380750.020.8725991
GGCGATC397550.019.829292
TCGCGTC194200.019.8047039
CCGATAA365150.019.603965
GTGGCGC1821400.019.4024983
AACCGAT376200.019.2563063
CTCGCGT197500.018.9196858
GGGGACC5370950.018.908577145
GGCGCGT1677950.018.5556415
CGAACCG417800.018.3085231
CGGAGGG1272350.018.010391
ACCGATA413850.017.7687424
CTACCGT306250.017.472388
GGGCGAT461750.017.4075151
Produced by FastQC (version 0.11.5)