FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787968

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787968
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102944137
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1236140.1200787180332572No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT271650.043.2043086
TCGACCG497500.041.35918145
AGACCGC327800.035.4486124
CCGCGTT334400.035.193767
GAACGTA374500.032.444055
CGCTATG430600.032.13653
AACGTAT387550.031.1513396
CGACCTA86750.031.083274
TCGCTAT451100.030.7160552
CAGACCG393750.030.4636673
CGTATTC452250.026.737458
GCGTTCT449150.026.0437879
CTCGCTA619000.025.0719151
GCGCTAT102200.022.8839171
CGATCCT1033800.022.403889145
AGAACGT341050.020.7803384
CTTCGAC792500.019.722803145
GTGCTCT1030950.019.339996145
GTCGCGT65100.018.918641
GGGTAGC641200.018.808126
Produced by FastQC (version 0.11.5)