FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787969

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787969
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences102944137
Sequences flagged as poor quality0
Sequence length35-151
%GC52

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC258400.024.3053111
TGCGACA311550.022.4155036
GCGACAT320650.022.1443927
TCGCGTC216850.022.0394389
CTCGCGT221900.021.4499558
TCAATCG186400.021.0699253
AGGGCGA787750.020.1406464
TGGCGCG888350.017.1205654
CGACATC427550.016.850998
AATCGCC381700.016.5588192
GCGAGGC932250.016.4538677
CGGTGGC1625600.016.4329871
GGCGATC285600.016.301972
CTACCGT199200.016.0927478
GTTTGAT838800.015.8844181
ACGCCCG979950.015.5865896
TACCGTA210650.015.5575669
TTGATCC809600.015.4206513
ACTACCG222350.015.0606087
GTGGCGC1170650.015.0027023
Produced by FastQC (version 0.11.5)