FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787971

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787971
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108205757
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG180350.026.5409283
TGGCGCG860400.024.5749154
GGGCGAT246750.024.3239141
GCGACAT376450.023.8124167
AGGGCGA733650.023.7924634
TGCGACA374050.023.7437136
GGCGATC266600.022.9873682
CAATCGC250200.022.3704431
CGGTGGC1484750.020.9736421
CGGAGGG850400.020.855931
GGTCACG474450.020.74495145
TACCGTA128100.019.2749619
GTGGCGC1187650.018.5772693
GGGGACC3529000.017.748745145
CGGCCGC2720300.017.500671145
GCGAGGC1039800.016.7880697
ACGCCCG834300.016.6943916
CGACATC548050.016.635378
GGGCGAG1106300.016.4422555
GAGGGCG1085450.016.422233
Produced by FastQC (version 0.11.5)