FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787972

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787972
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97241194
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1150850.11835004823161674No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1120300.11520837557794693No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1074320.11047992685075422No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1070100.11004595439253861No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA982140.10100040523977935No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG445300.050.6227145
CTCGCTA322350.041.317941
ACCGCGT212200.039.638466
TCGCTAT337950.038.7187732
CGCTATG358350.036.3545463
CCGCGTT247500.034.090357
AGACCGC243200.034.0495724
CAGACCG284600.029.6691783
ACCGCTA133100.028.5715352
CTTCGAC657600.027.98796145
CCGCTAA185650.022.96963
CGATCCT934800.021.831314145
GCGTTCT368050.021.0283059
CGTATCG108300.020.8915983
CCCGTAT190300.020.3427181
CTCGCGT506400.020.1889138
TCGCGTC508950.020.0749389
CGCGTTC417150.020.022468
CGCTAAG206150.019.5152154
CTCCGAT252100.019.2993221
Produced by FastQC (version 0.11.5)