FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787973

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787973
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences97241194
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA632550.028.217154
GCGACAT317150.025.6659477
CAATCGC253500.024.9658221
TGCGACA322700.024.8618286
TACCGTA144400.024.2776369
TGGCGCG672950.024.113714
TCAATCG177200.019.954933
GCGAGGC909400.019.918627
CTACCGT178900.019.707028
CGGTGGC1371550.019.5943431
CGACATC425700.019.304038
GGGCGAG974750.018.9013185
TCGCGTC162250.018.8110289
CGACCGT78150.018.6948761
CTCGCGT161600.018.6886088
GTGGCGC930350.018.4945833
GGCGAGG1009800.018.3159716
GGGGACC2991550.017.619612145
GGGCGAT260750.017.4876581
ACTACCG204750.017.0923677
Produced by FastQC (version 0.11.5)