FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787974

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787974
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87430009
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1202380.13752486288775287No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1125480.12872925587826486No Hit
CCCGGGTGCCCGGGCCCCCCTCGCGGGGGACCGTGCCCCCGCCGCCGGGG1117640.12783253859667337No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1098680.1256639468034368No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA980610.11215943029355058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG426500.061.02153145
ACCGCGT247550.058.7522626
CCGCGTT280550.051.79567
AGACCGC286050.050.8338744
CAGACCG348050.042.524983
CTCGCTA303150.038.1653561
GCGTTCT407050.035.8238649
TCGCTAT331600.034.621992
CGCTATG353950.032.0584343
CGCGTTC463000.031.3575948
GTGCTCT848900.030.953321145
ACCGCTA109300.030.6094072
GACCGCG519500.028.192085
CGATCCT785900.024.483803145
CCGCTAA162500.022.7802753
CTTCGAC590350.021.709068145
TCGCGTC402300.021.400649
CTCGCGT405150.021.312838
AGTCGCC660000.019.8220887
TCAGACC799100.019.8192652
Produced by FastQC (version 0.11.5)