FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787975

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787975
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences87430009
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG149050.026.0940763
TGCGACA266350.025.3194086
AGGGCGA484750.022.1891864
CAATCGC208950.020.7967241
GGTCACG393200.019.698904145
GCGACAT358900.019.1807867
CGACATC387150.017.9947018
TGGCGCG693400.017.3513054
TACCGTA121050.017.2185529
CGGTGGC1091850.016.536951
GCTGCGA419500.016.3236164
CTGCGAC431050.015.9998815
GGCGATC199400.015.9919092
GGGCGAT198700.015.7318471
ACGCCCG782850.015.5190436
CGGAGGG722700.015.2141581
AAGGGTC890750.014.564646145
GGGCGAG780250.014.2798865
GCGAGGC766550.014.1941427
CGGCCGC2324900.014.176168145
Produced by FastQC (version 0.11.5)