FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787976

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787976
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114245304
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1793560.15699201080510056No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1630050.1426798251593781No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1455580.12740830030090342No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1336110.11695097769620359No Hit
CCCCGCCGGGCCTTCCCAGCCGTCCCGGAGCCGGTCGCGGCGCACCGCCG1291550.11305059856114524No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGACCG430200.046.879368145
ACCGCGT338300.045.3119286
CTCGCTA504350.044.647711
TCGCTAT521200.042.8389432
CCGCGTT362800.042.2527
CGCTATG551900.040.16023
AGACCGC379600.040.1095434
CAGACCG422350.036.599943
ACCGCTA172800.029.1109262
GCGTTCT528100.029.001639
CGCGTTC568600.026.7841288
CTTCGAC645400.026.27946145
GACCGCG627900.024.47665
CGATCCT1225350.024.235146145
TTCGGCG525450.023.6139437
CCGCTAA234700.023.3688243
CGAGCCA636850.021.8953574
GTGCTCT1053750.021.720438145
GGATTCG594900.021.463734
TCGGCGA578600.021.4103728
Produced by FastQC (version 0.11.5)