FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787977

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787977
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114245304
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC332500.031.0628171
AGGGCGA728100.027.131584
TGGCGCG897800.026.09414
TCAATCG225950.024.6767313
TACCGTA156400.023.0123399
GCGACAT338050.022.4944927
GTGGCGC1193350.021.7781163
AATCGCC477200.021.7482992
TGCGACA345050.021.6718086
CGGTGGC2014750.020.8760051
CAACCCG808100.019.5276419
GCGAGGC1014200.019.397187
GGGCGAG1085900.018.835335
ACGATAC121650.018.1587185
CTACCGT201950.018.0852438
GGCGAGG1132550.018.0398676
GGCGATC330200.017.2171152
GGGGACC4459900.017.042843145
CGGAACG139950.016.7684821
GGGCGAT337150.016.4739671
Produced by FastQC (version 0.11.5)