FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787979

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787979
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences99845341
Sequences flagged as poor quality0
Sequence length35-151
%GC53

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1341210.13432875150378826No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1234820.12367327184550354No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA757600.032.889394
TGCGACA475100.029.764116
GCGACAT495000.028.8080127
GGGCGAT258750.027.8963661
TCAATCG179250.026.0617773
GGCGATC281850.025.5314542
CAATCGC256700.024.8436161
CGGAGGG1114500.023.0917931
CGACATC643200.022.3648018
TGGCGCG1155800.021.8422494
GCTGCGA672050.021.2480774
CGGTGGC1760200.021.0403561
GGGCGAG1258200.020.435885
CTGCGAC720650.020.0604065
GGCGAGG1308450.019.6318176
GAGGGCG1314750.019.4945983
GCGAGGC1273600.019.1566127
GTGGCGC1497000.018.496023
AATCGCC389900.016.6574972
GGGGACC4384500.015.300532145
Produced by FastQC (version 0.11.5)