FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787981

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787981
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences108791999
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1261980.11599933925288018No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCGACA333950.025.1021216
CGGTGGC1525850.023.7706991
GCGACAT380750.022.26427
AGGGCGA633750.020.617874
CAATCGC217000.020.356671
TGGCGCG1028150.020.1929044
TACCGTA185450.019.1951589
TCAATCG161450.018.6107353
CGACATC475400.017.9231388
GGCGATC254150.017.797652
GGGCGAT255500.017.4917371
GTGGCGC1316500.016.8730263
CTACCGT210350.016.592898
GCTGCGA519250.016.3142624
CTGCGAC536050.016.1562755
CGGAGGG855600.016.0935061
GGTGGCG2000600.015.7540772
GGGGACC4177850.015.368849145
GGCGCGT1293800.015.290595
CGGCCGC2690500.014.193795145
Produced by FastQC (version 0.11.5)