FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787982

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787982
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117076676
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1990750.170038138083114No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1594110.13615948577152978No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1467030.12530506076205988No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1415360.12089171373468102No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1336790.11418072716721135No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1296560.11074451755019078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT362650.051.846856
CCGCGTT398950.047.1293267
AGACCGC443200.042.4099924
CAGACCG465050.040.9199683
TCGACCG442350.038.149727145
CTCGCTA662400.037.7077451
CGCGTTC532050.035.2518588
GCGTTCT554700.034.043619
TCGCTAT737100.034.0195772
GTGCTCT1121500.032.19775145
GACCGCG601300.031.4534685
CGCTATG806500.031.16083
CGATCCT1543750.025.488356145
CTTCGAC613000.022.772108145
ACCGCTA182650.022.1238292
GCTATGT1242800.020.4391784
CCGCTAA212850.020.1984543
GTCTCGC727750.018.9059474
TCAGACC1081250.018.737522
AACGCTA455350.018.22235115-119
Produced by FastQC (version 0.11.5)