FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787984

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787984
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125736976
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2484430.19758945053680949No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1997900.15889518450006307No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1695260.1348258924248345No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1325200.1053946135940155No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1280210.10181650940929261No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT533150.060.277596
CCGCGTT577500.055.6161237
AGACCGC581400.054.803864
TCGACCG519850.052.971863145
CAGACCG622450.051.599223
GCGTTCT721150.044.4349
CTCGCTA503650.039.9501271
CGCGTTC852900.037.4606138
TCGCTAT549700.036.2060282
GACCGCG904900.035.6038975
CGCTATG602900.032.8211753
GTGCTCT1275500.032.321053145
ACCGCTA156600.026.5402972
TCAGACC1316700.025.6734732
CGATCCT1140100.025.461979145
CTCAGAC1620250.023.6933211
CTTCGAC741900.022.824778145
CCGCTAA231300.020.0139163
CGCTAGA274850.019.4652541
GTCGCGT53900.018.8228421
Produced by FastQC (version 0.11.5)