FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787985

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787985
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125736976
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1590790.1265172784177663No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC213250.024.727811
AGGGCGA721650.023.8295674
TGCGACA331000.023.2004326
GCGACAT343800.023.0818217
TCAATCG156050.022.260753
CGGAGGG873950.020.2769181
TGGCGCG898750.019.0574364
CGGTGGC1525500.018.3886811
CGGCCGC2630850.017.713968145
GGGCGAT245800.016.3735661
GGGCGAG1092050.016.3699555
GGCGATC245850.016.3675062
CGACATC494950.016.3233558
GGGGACC3660600.016.085938145
GAGGGCG1102000.015.92571453
GCGAGGC1085300.015.8384617
GGCGAGG1190850.015.274166
CTGCGAC522250.015.1350655
AATCGCC349800.015.0582542
ACGCCCG735500.014.8841366
Produced by FastQC (version 0.11.5)