FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787987

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787987
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences115590618
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2290090.1981207505958658No Hit
CGGAGGGCGAGGCCCGCGGCGGCGCCGCCGCCGCCGCGCGCTTCCCTCCG1747580.151187010696664No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGCGATC327750.031.070552
TCAATCG269950.030.86943
GGGCGAT330650.030.67551
AGGGCGA939700.030.4922984
CAATCGC406250.028.5605281
CGGAGGG1239750.023.781271
TACCGTA216000.022.4090219
CGGTGGC2411000.022.2113131
TGCGACA524350.021.843156
GCGACAT537750.021.3805667
GATCAAT446200.019.2891671
TGGCGCG1449050.019.1230344
GGGCGAG1572350.019.0942785
AATCGCC623700.018.9292472
GGGGACC5068650.018.203028145
CTACCGT269750.018.0137428
GGCGAGG1714750.017.7322336
ATCAATC528950.017.0402682
CGGCCGC5262300.016.881323145
GAGGGCG1756000.016.7437523
Produced by FastQC (version 0.11.5)