FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787989

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787989
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117054058
Sequences flagged as poor quality0
Sequence length35-151
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1756020.1500178661042234No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTGGC1535850.029.0426561
TGGCGCG1067900.024.5952154
GCGACAT423600.023.5540967
TGCGACA445950.022.160886
GTGGCGC1399800.022.1201363
CAATCGC222900.021.8299771
GGGCGAT266850.021.51731
GGTGGCG1842250.021.3081322
GGCGATC279850.020.2614022
GGCGCGT1124450.020.2527145
TCAATCG161900.019.503613
GGGGACC4455200.018.609081145
CGACATC553750.018.1206918
TACCGTA182100.018.0327789
AGGGCGA688100.016.6761444
GCTGCGA623350.016.0072754
CTGCGAC647350.015.6188785
GCGTGCC1446400.015.616918
CGTGCCT1510200.015.592919
CTACCGT218250.015.3640198
Produced by FastQC (version 0.11.5)