FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787990

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787990
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117473912
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT2304700.19618823964932744No Hit
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2283730.19440316246555234No Hit
CCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATCA1856140.15800444272256806No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1843720.15694718670814334No Hit
CCCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATAT1669120.14208431230246252No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACC1579500.13445538444314342No Hit
CCCTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATT1532570.13046045491359817No Hit
CTCCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGA1481430.12610714794277048No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1474780.12554106481105354No Hit
GCTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGG1436050.12224416260182089No Hit
CCTTAGGCAACCTGGTGGTCCCCCGCTCCCGGGAGGTCACCATATTGATG1405410.11963592393177475No Hit
CTCGCTATGTTGCTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATC1225580.10432784429618722No Hit
CTCAGGCTGGAGTGCAGTGGCTATTCACAGGCGCGATCCCACTACTGATC1176410.10014223413280048No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGACCGC587700.051.1172684
ACCGCGT600550.050.6956186
CAGACCG637000.047.571493
GTGCTCT1104350.047.25087145
CCGCGTT645400.047.2015467
ACCGCTA299150.046.746142
TCGACCG913850.040.962154145
CTCGCTA823000.038.4134861
GCGTTCT804500.037.511649
CCGCTAA425400.035.2083933
TCGCTAT931050.034.367942
CGCTATG1025500.031.2934343
CGCTAAG498700.030.1074814
CTTCGAC1196150.026.953794145
GTCTCGC766250.025.462044
GTCGCGT115900.025.0755961
AGAGTCG615450.024.1841649
CACCGCT616200.023.3149661
TCAGACC1376450.023.3101142
CGATCCT1882300.023.222456145
Produced by FastQC (version 0.11.5)