FastQCFastQC Report
Fri 22 Apr 2022
EGAF00005787991

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787991
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117473912
Sequences flagged as poor quality0
Sequence length35-151
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1419230.12081235534235041No Hit
CCGGCCCAGGTCGGAAACGGAGCAGGTCAAAACTCCCGTGCTGATCAGTA1180810.10051678537784628No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCAATCG242300.032.6796953
AGGGCGA884100.030.1155644
GGGCGAT296550.028.0697921
CAATCGC359900.027.9111421
GGCGATC312500.026.7018152
CGGAGGG1107600.024.3255181
GCGACAT480300.024.0591567
TACCGTA219800.023.8737329
TGCGACA506300.023.0605626
TGGCGCG1506600.023.0192074
CGGTGGC2380700.021.1294521
GTGGCGC1846300.020.1425173
GATCAAT422300.019.8931471
CTACCGT277750.019.252138
GAGGGCG1448200.018.7555543
GGGCGAG1480850.018.3690035
GGTCACG745950.018.358892145
AATCGCC559400.018.185472
CGACATC662300.017.80578
GGCGAGG1533650.017.7526426
Produced by FastQC (version 0.11.5)