FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787992

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787992
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119423897
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA1505520.1260652212680683No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1504310.1259639015129443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT401300.050.2624866
CCGCGTT442850.045.5187577
AGACCGC438350.045.421354
TCGACCG445550.043.650223145
CAGACCG493000.040.8540763
CTCGCTA412200.037.8464741
GCGTTCT576800.034.787359
TCGCTAT442650.034.7513352
ACCGCTA184900.034.6616252
GTGCTCT1178950.033.773148145
CGCTATG468900.032.6733933
CGCGTTC706850.028.290888
GACCGCG744500.027.1505225
CCGCTAA262300.025.9645773
CTTCGAC637950.024.159105145
CGCTAAG310350.021.6836134
CGATCCT797600.021.21111145
CGGTGCT722900.019.150484145
CGTATCG77500.018.899253
TCGTACG197800.018.72113610-14
Produced by FastQC (version 0.11.5)