FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787993

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787993
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119423897
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1474100.12343425704823549No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGCGA678050.023.7960954
CAATCGC219800.022.1296621
CGGAGGG765500.021.8956051
TCAATCG158350.021.6103483
TGCGACA305500.018.4499556
GCGACAT310600.018.3867597
GGCGATC245300.017.7245042
GGGCGAT240300.017.5289651
CGGCCGC2287800.017.507742145
TACCGTA149000.016.1229139
GAGGGCG1025700.016.1000123
GGGCGAG1033150.015.6938825
GCGAGGC1025050.015.6422127
TGGCGCG725550.015.5787374
GGCGAGG1090350.015.2804876
AATCGCC338400.014.426662
ACGCCCG636500.013.7512246
CGACATC428550.013.6738028
CGGTGGC1339950.013.6127271
GCCGTAT225200.013.5656395145
Produced by FastQC (version 0.11.5)