FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787994

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787994
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113138096
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2272740.2008819381227699No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1654990.14628052428953728No Hit
CTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAAATGGACCTT1485530.1313023687441231No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT553650.060.9667936
CCGCGTT594750.056.700347
AGACCGC605800.055.62424
CAGACCG646950.052.517843
TCGACCG603650.052.136772145
GCGTTCT733500.045.9142469
CGCGTTC793250.042.4158368
GACCGCG800450.042.2484055
GTGCTCT1232050.038.369938145
CTCGCTA319400.034.216851
TCGCTAT342300.031.6377742
CGCTATG367300.029.1696933
TCAGACC1295400.027.3833312
ACCGCTA167700.026.8378012
CTTCGAC803200.026.670883145
CTCAGAC1563150.025.5849191
CCGCTAA221750.022.411893
CGATCCT723950.021.440912145
CGGTGCT858100.020.301292145
GCGGACG1061300.019.676167145
Produced by FastQC (version 0.11.5)