FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787995

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787995
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences113138096
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1573630.13908931258662863No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC271900.028.6236821
TGCGACA354500.025.7318046
TCAATCG179400.024.962863
GCGACAT372200.024.8340577
AGGGCGA658250.021.3890424
AATCGCC401800.019.5154652
GGCGATC266800.018.9313282
CGACATC508500.018.3530658
GGGCGAT274650.018.2721251
CTGCGAC540600.017.004885
CGGAGGG903200.016.3685251
GCTGCGA570150.016.134524
AGGGATC734300.015.9483682
TGGCGCG685350.014.734944
GCGAGGC955400.014.6489697
CGGCCGC2275800.014.628458145
GGGCGAG996550.014.512365
GGGATCA812900.014.0159983
TACCGTA149350.013.9241859
GGCGAGG1039950.013.838126
Produced by FastQC (version 0.11.5)