FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787996

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787996
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124750977
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCAGACCGCGTTCTCTCCCTCTCACTCCCCAATACGGAGAGAAGAACGA2825110.22645994988880927No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1377230.11039833379421149No Hit
CTCCGTTTCCGACCTGGGCCGGTTCACCCCTCCTTAGGCAACCTGGTGGT1366620.10954783945299282No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGCGT627050.060.8969656
AGACCGC677900.056.1449624
CCGCGTT691450.055.2432567
CAGACCG732300.052.6061063
GTGCTCT1333200.047.51608145
GCGTTCT836700.045.5855759
TCGACCG314750.039.331444145
CTCGCTA506050.037.8476371
CGCGTTC1061550.035.8062488
TCGCTAT555850.034.1689532
GACCGCG1151550.033.3286555
CGCTATG606850.031.3076783
TCAGACC1413750.028.4744532
ACCGCTA196350.027.8690172
CTCAGAC1728950.025.857911
CGATCCT1113350.023.73764145
CCGCTAA249350.022.9235213
AACGCTA758650.020.112677115-119
GTCGCGT94250.019.952291
ACGCTAC744750.019.517931115-119
Produced by FastQC (version 0.11.5)