FastQCFastQC Report
Wed 20 Apr 2022
EGAF00005787997

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00005787997
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124750977
Sequences flagged as poor quality0
Sequence length35-151
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1287380.1031959853909601No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAATCGC328850.028.3680921
TCAATCG228400.028.2617993
AGGGCGA839050.020.4774234
AATCGCC497500.019.0994262
CGGAGGG987550.018.412361
TACCGTA172100.018.1844759
GGTCACG711700.016.848614145
TGCGACA264300.015.1222736
CGGTGGC1798750.015.0782031
AGGGATC754000.015.070782
GCGACAT276200.014.5844987
GGGGACC3389550.014.497242145
CTACCGT225350.014.3058328
TGGCGCG975650.014.2416194
CGGCCGC3368050.014.128582145
GGGCGAG1285500.013.9573925
GATCAAT506250.013.9200351
ATATCGG248450.013.876816145
ATCAATC530800.013.6125052
GCGAGGC1257400.013.5291957
Produced by FastQC (version 0.11.5)